Optimization Genome Editing Tools
- Importance of the Research Topic
The journey of monoclonal antibody drugs from mouse-derived Muromonab-CD3 in 1986 to fully humanized Panitumumab in 2006 exemplifies the power of continuous innovation and refinement. Similarly, genome editing platform technologies have been steadily evolving. At GRIT-Lab, we are committed to advancing these tools to ensure they become ‘best in class’ solutions, and focus on enhancing the precision, efficiency, and safety of genome editing tools to meet and go beyond the stringent demands of the competitive market.
- Our Research
At GRIT-Lab, we employ a directed evolution approach to optimize various types of genome editing tools. For example, while he was at Toolgen, Dr. Lee patented ‘Sniper-screen,’ which can simultaneously select clones of CRISPR mutants with high activity and high specificity. The latest Cas9 mutant, named ‘Sniper2L‘, demonstrates higher specificity while retaining activity, marking it as an outlier to the typical trade-off relationship between general activity and specificity found in high-throughput studies.. Additionally, single-molecule FRET experiments have confirmed its enhanced specificity and maintained activity.
Leveraging our experience in directed evolution, GRIT-Lab plans to further optimize various genome editing tools to maximize their efficiency and safety.
